How to choose the best reagents for pluripotent stem cells
When working with pluripotent stem cells, what should you use for each step of your workflow? What medium should you use to reprogram blood cells? What about fibroblasts? What medium should you use to grow human pluripotent stem cells (hPSCs)? How are each of the step-phase reactants different from each other? Should you plate hPSCs on Corning® Matrigel® or Vitronectin XF™? According to what criteria should you choose a cryopreservation medium?
There are many things to consider when choosing reagents for each stage of your workflow. StemCell Technologies has provided some outstanding infographics on the site
Reprogramming
Which TeSR™ media should you use for feederless reprogramming?
Induced pluripotent stem (iPS) cells are derived from somatic cells through the artificial expression of transcription factors
key under specific growing conditions. Variables affecting reprogramming include delivery method, culture conditions, and starting cell type. Use this infographic to choose the ideal terrain
Passaging
Which reagent to choose?
From enzymatic preparation to enzyme-free reagents, single cell generation to selective detachment of undifferentiated cells – there are multiple options that eliminate the variability associated with hand sorting and scraping during steps. Use this infographic to choose the optimal reagents for your needs
Which TeSR™ medium should you use for culturing hPSCs?maintenance
Maintaining high-quality human embryonic stem (ES) and induced pluripotent stem (iPS) cells is critical to the success of PSC research. Specific culture conditions are required to maintain undifferentiated ES and iPS cells capable of self-renewal. Use this infographic to choose the best growing medium for your needs
Right matrix for plate coating
Which matrix should you choose for hPSC culture?
From defined and xenon-free matrices to more complex matrices, a selection of matrices is available that reduces preparation time and variables in research. Use this infographic to choose the best matrix for your needs
Cryopreservation
Which cryopreservation medium should you use?
From animal-free media to those for freezing cell aggregates or single cells, the formulations available are designed to maintain high viability and maximize cell culture recovery after thawing. Use this infographic to choose the optimal wording for your needs
